使用密度梯度 density gradient centrifugation來分
使用密度梯度 density gradient centrifugation來分離狗精子性別的技術並不有效。
Anim Reprod Sci
. 2018 Dec:199:84-92.
Sperm sexing with density gradient centrifugation in dogs
Sperms were separated using gradients of Percoll® or Ficoll.
The percentage of sperm containing X and Y chromosomes was evaluated by qPCR.
The use of the Ficoll gradient resulted in the greatest sperm quality after centrifugation.
No sperm enhancement containing X or Y chromosome occurred with use of any of the methods
Percoll® 54.8 ± 1.9 compared with 45.2 ± 1.9;
Percoll® associated with Nycodenz® 53.2 ± 2.0 compared with 46.8 ± 2.0;
Ficoll 55.0 ± 1.5 compared with 45.0 ± 1.5
for the percentages of cells containing the X and Y chromosomes, respectively
The technique of sexing dog sperm using density gradients was not effective for commercial application.